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Immobilization of chymotrypsin with interpolymer complexes of P(TM‐ co ‐AAm)/PAA
Author(s) -
Wang Hongzuo,
Liu Shiyong,
Wang Ying
Publication year - 2001
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.1633
Subject(s) - cationic polymerization , chymotrypsin , thermal stability , polymer chemistry , chemistry , acrylamide , ammonium chloride , polymer , acrylic acid , polyelectrolyte , immobilized enzyme , copolymer , enzyme , trypsin , organic chemistry
Chymotrypsin was immobilized with interpolymer complexes formed by the cationic polymer poly(allyltrimethyl ammonium chloride‐ co ‐acrylamide) [P(TM‐ co ‐AAm)] and poly(acrylic acid) (PAA). The introduction of a small amount of cationic groups led to a much stronger polymer–polymer interaction between P(TM‐ co ‐AAm) and PAA. The characteristic pH sensitivity of this kind of complex provided the possibilities of controlling the activity of the immobilized enzyme and separating the immobilized enzyme from the batch by changing the pH of the medium. Compared with the free enzyme, the immobilized chymotrypsin had higher thermal stability, acid–base stability, and stability in use. © 2001 John Wiley & Sons, Inc. J Appl Polym Sci 81: 2013–2018, 2001