Premium
Characterization and application of high magnetic property chitosan particles
Author(s) -
An Xiaoning,
Su Zhixing
Publication year - 2001
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.1539
Subject(s) - glutaraldehyde , chitosan , epichlorohydrin , magnetic nanoparticles , materials science , trypsin , chemical engineering , aprotinin , polymer chemistry , chemistry , chromatography , nanotechnology , nanoparticle , organic chemistry , enzyme , medicine , surgery , engineering
High magnetic property chitosan particles were prepared from chitosan coated on magnetic powder, glutaraldehyde modified, and then epichlorohydrin crosslinked. The magnetic properties, the surface, and the morphology of the magnetic particles were characterized. An optimum condition of immobilization of aprotinin on magnetic particles was obtained, then these particles were used for affinity purification of trypsin, and the condition of affinity purification was discussed. The proposed method was successfully applied to the purification of trypsin. © 2001 John Wiley & Sons, Inc. J Appl Polym Sci 81: 1175–1181, 2001