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Gelatin microspheres crosslinked with γ‐ray: Preparation, sorption of proteins, and biodegradability
Author(s) -
Terao Ken,
Karino Tomotake,
Nagasawa Naotsugu,
Yoshii Fumio,
Kubo Miyoko,
Dobashi Toshiaki
Publication year - 2004
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.13574
Subject(s) - gelatin , sorption , materials science , fluorescence , microsphere , biodegradation , chemical engineering , adsorption , swelling , chemistry , polymer chemistry , nuclear chemistry , composite material , organic chemistry , physics , quantum mechanics , engineering
A new type of gelatin microspheres was manufactured with the crosslinking method by using γ‐ray irradiation. Microspheres thus obtained were characterized by microscopic observation and enzymatic degradability. The mean diameter of the microspheres was in the range from 2.4 to 3.6 μm and the size distribution was quite small. The size decreased with increasing the shear rate of the emulsifier used in the preparation and was not affected by the radiation dose. The enzymatic degradability decreased with increasing the radiation dose and decreasing the gelatin concentration of the microsphere. In other words, the rate of the enzymatic decomposition of microspheres can be controlled by these two parameters. These trends were consistent with our previous results for the gel sheet made of gelatin. Their sorption of proteins was also investigated with the use of three types of proteins labeled by fluorescent pigment. From the fluorescence micrographs, it was observed that the gelatin microspheres adsorbed only proteins having the opposite charge. © 2004 Wiley Periodicals, Inc. J Appl Polym Sci 91: 3083–3087, 2004