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Immobilization of glucose oxidase in chitosan gel beads
Author(s) -
Ghanem Amyl,
Ghaly Ahmed
Publication year - 2003
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.13221
Subject(s) - carbodiimide , glucose oxidase , immobilized enzyme , chitosan , biopolymer , chemistry , enzyme , conjugate , enzyme assay , biosensor , chromatography , polymer chemistry , polymer , biochemistry , organic chemistry , mathematical analysis , mathematics
Glucose oxidase was conjugated to the biopolymer chitosan using carbodiimide conjugation. Gel beads were formed from the enzyme–biopolymer complex, and the activity of the immobilized enzyme was compared to the method of encapsulating the enzyme in chitosan. The activity of the immobilized enzyme was found to increase with increasing carbodiimide concentration and time of incubation, to a maximum. Enzyme activity decreased with time as the beads were air‐dried. The pH optima of the immobilized enzyme was shifted to a more acidic value compared to that of the free enzyme. The use of carbodiimide chemistry to conjugate glucose oxidase to a chitosan gel was demonstrated. This technique holds promise for the development of immobilized enzymes for applications in medicine, biosensors, and bioprocessing. © 2003 Wiley Periodicals, Inc. J Appl Polym Sci 91: 861–866, 2004