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Effect of purified Trichoderma reesei cellulases on formation of cotton powder from cotton fabric
Author(s) -
Heikinheimo Lea,
MiettinenOin Arja,
CavacoPaulo Artur,
Buchert Johanna
Publication year - 2003
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.12868
Subject(s) - cellulase , trichoderma reesei , chemistry , biodegradation , mode of action , enzyme , polymer chemistry , trichoderma , food science , materials science , chemical engineering , biochemistry , organic chemistry , horticulture , biology , engineering
The mode of action of monocomponent purified Trichoderma reese i cellobiohydrolases (CBHI and CBHII) and endoglucanases (EGI and EGII) on cotton fabrics was studied by analyzing the weight loss of the fabric, the reducing sugars, the soluble oligosaccharides and the molecular weight of the cotton powder formed. The impact of mechanical action on these factors was also evaluated. EGI and EGII released the highest amounts of reducing sugars and soluble oligosaccharides in both treatments with or without additional mechanical action. After cellulase treatment without additional mechanical action, all of the cellulases were found to have reduced the molecular weight of cotton poplin powder. When mechanical action was combined with enzyme treatments, only EGII reduced the molecular weight. The weight loss of EG‐treated fabrics was clearly higher than the weight loss of CBH‐treated fabrics with both low and high mechanical action levels. © 2003 Wiley Periodicals, Inc. J Appl Polym Sci 90: 1917–1922, 2003