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Preparation and characterization of poly(dimethyldiallyl ammonium)chloride and antiglobulin tests for antibody detection
Author(s) -
Yang Xujie,
Wang Xin,
Wang Hengzhi,
Zhu Junwu,
Lu Lude,
Zhou Tongbin,
Lian Jianfeng
Publication year - 2003
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.11549
Subject(s) - polyelectrolyte , monomer , ammonium chloride , nuclear chemistry , polymer , polymer chemistry , bromide , chemistry , ammonium bromide , characterization (materials science) , materials science , nanotechnology , organic chemistry , biochemistry , pulmonary surfactant
Poly(dimethyldiallyl ammonium)chloride [poly(DMDAAC)] was prepared in our laboratory and monomer‐to‐polymer structures were characterized by infrared (IR), proton, and carbon‐13 nuclear magnetic resonance (NMR) as well as DEPT techniques. The polyelectrolytes were used to detect the antibodies of red blood cells (RBCs) in human sera and the results were compared with hexadimethrine bromide (polybrene), bromeline, and other methods. This article presents data supporting the use of the manual poly(DMDAAC) test; the 1‐min procedure at room temperature provided a rapid and sensitive test. The states of agglutination of RBCs were investigated by transmission electron microscopy (TEM). The results of the morphology were in agreement with the manual poly(DMDAAC) test. © 2003 Wiley Periodicals, Inc. J Appl Polym Sci 87: 1957–1961, 2003