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New supports for enzyme immobilization based on copolymers of vinylene carbonate and β‐hydroxyethylene acrylate
Author(s) -
Ding Lunhan,
Li Yue,
Jiang Yan,
Cao Zhe,
Huang Jiaxian
Publication year - 2001
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.10036
Subject(s) - copolymer , polymer chemistry , acrylate , trypsin , chemistry , immobilized enzyme , aqueous solution , carbonate , nuclear chemistry , materials science , organic chemistry , enzyme , polymer
Vinylene carbonate (VCA), β‐hydroxyethylene acrylate, and N,N ′‐methylene bisacrylamide were dissolved in water, and the aqueous solutions were copolymerized via reverse‐phase suspension copolymerization in paraffin oil. A series of hydrophilic and beaded supports containing reactive cyclic carbonate groups for enzyme immobilization were obtained. The supports were examined by coupling with trypsin, and the results showed that the amount of enzymes coupled to the supports and the specific activity of the immobilized trypsin were related to the content of VCA structure units and the reaction time. Meanwhile, the optimal pH and temperature, as well as the Michaelis–Menten constant K m , for both native and immobilized trypsin were measured. © 2002 Wiley Periodicals, Inc. J Appl Polym Sci 83: 94–102, 2002