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Response Surface Methodology, an effective strategy in the optimization of the generation of curcumin‐loaded micelles
Author(s) -
Wei Tan Khang,
Manickam Sivakumar
Publication year - 2012
Publication title -
asia‐pacific journal of chemical engineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.348
H-Index - 35
eISSN - 1932-2143
pISSN - 1932-2135
DOI - 10.1002/apj.661
Subject(s) - curcumin , dispersity , curcuma , response surface methodology , pulmonary surfactant , bioavailability , zeta potential , particle size , sonication , micelle , central composite design , solubility , chromatography , box–behnken design , chemistry , materials science , aqueous solution , chemical engineering , nanoparticle , nanotechnology , organic chemistry , botany , bioinformatics , biochemistry , biology , engineering
Curcumin, a yellow pigment extracted from the dried rhizome of Curcuma longa , found to have significant pharmaceutical activities. However, the bioavailability of curcumin after administration is always low because of its extremely poor solubility in water. To improve the absorption rate of curcumin in vivo , curcumin‐loaded micelles have been developed by using GRAS surfactants, namely Brij 56 and Span 20, and by the application of ultrasonic cavitation. To investigate the interaction between different factors and to optimize the formulation conditions, Response Surface Methodology has been adopted. Both Central Composite Design and Box–Behnken Design (BBD) have been attempted in this study, and a better fitted quadric polynomial equation has been obtained from BBD that resulted into the coefficient of determination ( R 2 ) of 0.9894 and 0.9574 for the zeta‐average and polydispersity index of the formulation, respectively. In contrast, Analysis of Variance suggested a coefficient of determination ( R 2 ) for Central Composite Design plots that is 0.9172 for particle size response surface and 0.8246 for the polydispersity response surface. Optimization based on BBD model suggested 28 possible solutions, for which the generated solution with the highest desirability (0.764) predicted the curcumin‐loaded micelle formulation with 19.99 nm particle size and with 0.267 polydispersity index, when the formulation hydrophilic–lipophilic balance number is 9.46, curcumin‐to‐water weight ratio is 0.7 and surfactant‐to‐water weight ratio is 0.11. The whole investigation clearly supports the potential of ultrasonic cavitation in the generation of curcumin‐loaded micelles. © 2012 Curtin University of Technology and John Wiley & Sons, Ltd.