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Callus Induction from Tobacco (Nicotiana tabacum) Leaf Explants for the Production of Quinone
Author(s) -
Husin Murnilawati Abd Gani,
Hasan Masitah,
Taha Rosna Mat
Publication year - 2005
Publication title -
developments in chemical engineering and mineral processing
Language(s) - English
Resource type - Journals
eISSN - 1932-2143
pISSN - 0969-1855
DOI - 10.1002/apj.5500130506
Subject(s) - callus , kinetin , nicotiana tabacum , explant culture , botany , darkness , murashige and skoog medium , horticulture , biology , tobacco leaf , chemistry , in vitro , biochemistry , gene , agricultural engineering , engineering
This paper reports the growth characteristics, measurement of fresh weight and dry weight of callus of tobacco (Nicotiana tabacum) cells grown on Murashige and Skoog (MS) medium supplemented with 2 mg/L NAA (naphtaleneacetic acid), 0.2 mg/L kinetin and 0.2 mg/L 2, 4‐D (2,4‐dichlorophenoxyacetic acid) at 25d̀C, under 16 hours light and 8 hours darkness. Leaf explants obtained from 3‐5 month old intact plants of tobacco were used to initiate cultures. MS medium supplemented with 2.0 mg/L NAA together with 0.2 mg/L kinetin gave rise to optimum callus induction.
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