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Mouse out root sheath cells cultured on E‐cadherin‐coated dishes exhibit neuronal cell morphology
Author(s) -
Xia Lingling,
Liu Qing,
Zhou Guangdong,
Zhang Wenjie,
Cao Yilin,
Liu Wei
Publication year - 2011
Publication title -
asia‐pacific journal of chemical engineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.348
H-Index - 35
eISSN - 1932-2143
pISSN - 1932-2135
DOI - 10.1002/apj.467
Subject(s) - cadherin , microbiology and biotechnology , embryonic stem cell , cell culture , nestin , cell , stem cell , biology , cellular differentiation , downregulation and upregulation , chemistry , neural stem cell , gene , biochemistry , genetics
Epithelial cadherin (E‐cadherin) coating system has been employed to culture embryonic undifferentiated cells in order to better maintain stem‐cell self‐renewal function and cell proliferation potential. In this study, recombinant E‐cad‐Fc was used to coat the dish at a concentration of 10 µg/ml to observe the effect of the coating on cell behavior of cultured mouse out root sheath (ORS) cells. The results demonstrated that culturing the ORS cells on E‐cadherin‐coated dishes could lead to neuronal cell morphology change of part of the ORS cells. In addition, Nestin gene expression was upregulated in the cells of coated group compared to the cells of non‐coated group. Particularly, elongated cell morphology with fine cell processes connecting to nearby cells became significant in the subpopulation of the cells that strongly adhered to the coated culture dish. These results preliminarily indicate that E‐cadherin coating may favor the neurogenic lineage differentiation of hair follicle derived cells, which deserves further detailed studies. © 2010 Curtin University of Technology and John Wiley & Sons, Ltd.