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Immobilized glucose oxidase biofuel cell anode by MWCNTs, ferrocene, and polyethylenimine: Electrochemical performance
Author(s) -
Bahar Tahsin,
Yazici M. Suha
Publication year - 2017
Publication title -
asia‐pacific journal of chemical engineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.348
H-Index - 35
eISSN - 1932-2143
pISSN - 1932-2135
DOI - 10.1002/apj.2149
Subject(s) - polyethylenimine , glucose oxidase , anode , electrochemistry , ferrocene , cyclic voltammetry , chemistry , immobilized enzyme , nafion , nuclear chemistry , electrode , chemical engineering , materials science , organic chemistry , enzyme , biochemistry , transfection , engineering , gene
Ferrocene‐functionalized polyethylenimine and multiwalled carbon nanotubes were attached covalently by glutaraldehyde onto a carbon cloth to develop an immobilized enzyme (glucose oxidase) electrode for biofuel cell applications. Developed enzymatic anode was characterized by electrochemical methods to determine electrochemical performance. Anodic open‐circuit potential was measured as within 0–20 mV range. Cyclic voltammetry showed anodic peak for glucose oxidation around 400–600 mV (vs. sat. Ag/AgCl) varying with scan rate. An enzyme fuel cell with 2.5 mg/cm 2 glucose oxidase‐loaded bioanode and 0.70 mg/cm 2 Pt‐loaded cathode attached to Nafion™ 115 membrane has provided around 2.5 mA/cm 2 current density at short‐circuit conditions. Enzymatic kinetic parameters of prepared anode were determined by electrochemical methods that surprisingly indicated less K M (i.e., better substrate affinity) than that of determined by conventional enzymatic methods. Enzymatic stability determined by electrochemical methods moreover indicated longer enzyme half‐life.