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Surfactant‐assisted separation of ginkgo flavonoids from Ginkgo biloba leaves using leaching and foam fractionation
Author(s) -
Jiao Meng,
Wu Zhao Liang,
Liu Yan,
Liu Wei,
Li Rui
Publication year - 2016
Publication title -
asia‐pacific journal of chemical engineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.348
H-Index - 35
eISSN - 1932-2143
pISSN - 1932-2135
DOI - 10.1002/apj.1992
Subject(s) - foam fractionation , pulmonary surfactant , fractionation , chemistry , chromatography , ginkgo , leaching (pedology) , sodium dodecyl sulfate , ginkgo biloba , botany , biochemistry , environmental science , soil water , soil science , biology
A surfactant can be used as solubilizer in leaching or collector in foam fractionation. However, at the present, there are not any references on the two synergetic effects of it for improving the separation efficiency of foam fractionation. So in this work, a surfactant‐assisted foam fractionation technology was developed to separate ginkgo flavonoids (GF) from the leaching solution of Ginkgo biloba leaves, where the surfactant played the dual roles of solubilizer and collector. The solubilization mechanism of GF by the surfactant of sodium dodecyl sulfate was mainly attributed to electrostatic attraction and hydrogen bonds. The GF concentration reached 1.13 mg/mL under the optimal leaching conditions determined by response surface methodology. A two‐stage foam fractionation technology was developed for effectively enriching GF from the leaching solution, where the residual solution of the first stage was used as the loading solution of the second stage and the foamate of the second stage was used as the feeding solution of the first stage. Both the enrichment ratio and the recovery percentage of GF were improved, and they were 5.82% and 76.25%, respectively, under the optimum conditions. Finally, seven ingredients of GF in the foamate were identified by using HPLC‐MS/MS. © 2016 Curtin University of Technology and John Wiley & Sons, Ltd.