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Extraction and fractionation of polyunsaturated fatty acids from Mortierella sp. using supercritical fluid: experimental and kinetic studies
Author(s) -
Tilay Ashwini,
Azargohar Ramin,
Dalai Ajay K.,
Annapure Uday S.,
Kozinski Janusz A.
Publication year - 2014
Publication title -
asia‐pacific journal of chemical engineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.348
H-Index - 35
eISSN - 1932-2143
pISSN - 1932-2135
DOI - 10.1002/apj.1779
Subject(s) - supercritical fluid , polyunsaturated fatty acid , fractionation , extraction (chemistry) , chemistry , supercritical fluid extraction , yield (engineering) , chromatography , solvent , biomass (ecology) , supercritical carbon dioxide , fatty acid , organic chemistry , materials science , agronomy , biology , metallurgy
The growing interest in various applications of polyunsaturated fatty acids daily dietary requirements and demand has directed much attention on the industrial production of bio‐oil from plant, animal, fungi, or algae sources. This work signifies that the extraction of polyunsaturated fatty acids from fungal biomass of Mortierella sp. 1458 using supercritical CO 2 (SC‐CO 2 ) with subsequent fractionation of various fatty acids present in biomass using co‐solvents was investigated. The use of co‐solvent in supercritical fluid extraction has provided the green system as a substitute for classical solvent extraction at industrial scale. The optimized extraction temperature (62.3 °C), pressure (298.5 bar), and time (88.6 min) on SC‐CO 2 extraction conditions gave maximum recovery up to 66.35% of bio‐oil from dried biomass. Further co‐solvent addition to SC‐CO 2 has improved % recovery up to 97.88%. The bio‐oil obtained by this method was examined for use by characterization with value added chemicals such as omega‐3, omega‐6, and omega‐9 fatty acids. Peleg's equation was used to study the bio‐oil extraction kinetics. The equilibrium yield as well as the time required to reach half of equilibrium yield were also calculated. It showed that for an effective extraction process with high yield, CO 2 density larger than 700 kg/m 3 is needed. © 2014 Curtin University of Technology and John Wiley & Sons, Ltd.