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A gas chromatography–mass spectrometry method for the detection of chlorpyrifos contamination in palm‐based fatty acids
Author(s) -
Tay Bonnie Yen Ping,
Wai Weng Hong
Publication year - 2021
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1002/aocs.12512
Subject(s) - capric acid , chromatography , chemistry , detection limit , chlorpyrifos , calibration curve , gas chromatography–mass spectrometry , acceptable daily intake , contamination , matrix (chemical analysis) , mass spectrometry , fatty acid , pesticide , biochemistry , biology , ecology , agronomy , lauric acid
Abstract Chlorpyrifos is a Malaysian Pesticide Board‐approved organophosphate insecticide, which may become concentrated during fractionation. The objective of this project was to develop and validate a method to detect and quantify chlorpyrifos in food‐grade fatty acids ingredients, e.g. caprylic‐capric acid mixture and oleic acid (OLA) used to synthesize triacylglyceride based food additives and in the cosmetic industry. A selective ion monitoring gas chromatography–mass spectrometry method with a matrix‐matched calibration curve calibration was selected. The method involved the direct injection of chlorpyrifos spiked into the fatty acids matrix. The percentage recoveries at spiking levels of 0.5, 0.75, 2.5, and 4.0 μg g −1 of chlorpyrifos in OLA and caprylic‐capric acid ranged from 85.7%–101.1% to 97.2%–112%, respectively, with a relative standard deviation of within 11%. The intra‐day and inter‐day precision were deemed acceptable as indicated by an relative SD value of within 10%. A good linear relationship with a coefficient of correlation >0.99 for the matrix‐matched calibration was achieved between 0.5 and 5 μg g −1 . The limit of detection and limit of quantification corresponded to 0.5 μg g −1 for OLA and 0.55 μg g −1 for caprylic‐capric acid.