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Fatty Acid Profile and Bioactive Compound Extraction in Purple Viper's Bugloss Seed Oil Extracted with Green Solvents
Author(s) -
RincónCervera Miguel Ángel,
GalleguillosFernández Raúl,
GonzálezBarriga Valeria,
Valenzuela Rodrigo,
Speisky Hernán,
Fuentes Jocelyn,
Valenzuela Alfonso
Publication year - 2020
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1002/aocs.12328
Subject(s) - chemistry , oxygen radical absorbance capacity , extraction (chemistry) , solvent , chromatography , ethyl acetate , hexane , polyphenol , phytosterol , fatty acid , ethanol , methanol , organic chemistry , antioxidant , dpph
Oil extraction from seeds of purple viper's bugloss ( Echium plantagineum ) was carried out using different solvents (chloroform:methanol, n‐hexane, ethanol, 2‐propanol and ethyl acetate) at room temperature and also using Randall extraction. Extraction yields were calculated and oils were analyzed in terms of fatty acid profiles and distribution among lipid classes, total polyphenol content, oxygen radical absorbance capacity (ORAC) and phytosterol content. No considerable differences were found on fatty acid profiles and distribution in oils regardless of the solvent and temperature used for the extraction. However, ethanol combined with Randall extraction (85 °C for 1 hour) offered the best results in terms of total polyphenol content (20.9 mg GAE/100 g oil), ORAC (468.0 μmol TE/100 g oil), and phytosterol amount (437.2 mg identified phytosterols/100 g oil) among all assayed extraction methods. A higher extraction temperature led to significantly higher concentrations of bioactive compounds and ORAC values in the oil when ethanol or 2‐propanol were used as extracting solvent, but that was not the case using n‐hexane except for the concentrations of β‐sitosterol and stigmasterol, which were significantly higher using Randall extraction than room temperature extraction with n‐hexane. Ethanol is classified as a “green solvent,” and it could be considered a suitable option to produce oil from E. plantagineum seeds with a higher antioxidant capacity and bioactive compound concentration than the current commercial oil, which is usually extracted with n‐hexane.