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Estimation of Protein and Fatty Acid Composition in Shell‐Intact Cottonseed by Near Infrared Reflectance Spectroscopy
Author(s) -
Wang Qingkang,
Xing Huixian,
Liu Xiangliu,
Mao Lili,
Wei Ze,
Zhang Haijun,
Wang Liyuan,
Wang Haoran,
Saeed Muhammad,
Zhang Guihua,
Song Xianliang,
Sun XueZhen,
Yuan Yanchao
Publication year - 2020
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1002/aocs.12312
Subject(s) - cottonseed , near infrared reflectance spectroscopy , stearic acid , partial least squares regression , chemistry , fatty acid , myristic acid , linoleic acid , palmitic acid , oleic acid , composition (language) , conjugated linoleic acid , chromatography , food science , near infrared spectroscopy , biochemistry , biology , mathematics , organic chemistry , statistics , linguistics , philosophy , neuroscience
Rapid and accurate analysis of cottonseed protein content and the composition of fatty acids (especially, saturated fatty acids) is often required in cotton production and breeding programs. This study aimed to establish a set of effective estimation models for these parameters. Near infrared reflectance spectroscopy (NIRS) calibration equations using partial least‐squares regression for protein concentration, oil concentration, and five fatty acids of shell‐intact cottonseeds were established based on 90 varieties, and the prediction abilities of the calibration models were verified using 45 other varieties. The prediction abilities of the NIRS calibration equations were basically consistent with external validation results. Each equation was assessed based on the ratio of performance to deviation (RPD p ). Protein content and seed total fatty acid (STA) content had high RPD p values (3.687 and 3.530, respectively), whereas cottonseed kernel total fatty acid (KTA) content, linoleic acid (18:2), stearic acid (18:0), myristic acid (14:0), and palmitic acid (16:0) exhibited relatively high RPD p (2.866, 2.836, 2.697, 2.676, and 2.506, respectively). The calibration model for oleic acid (18:1) had a low RPD p (1.945). The results indicated that NIRS can be used to rapidly determine contents of STA, KTA, protein, stearic acid (18:0), myristic acid (14:0), and palmitic acid (16:0) in shell‐intact cottonseed.