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Production of 6,8‐Dihydroxy Fatty Acids by Recombinant Escherichia coli Expressing T879A Variant 6,8‐Linoleate Diol Synthase from Penicillium oxalicum
Author(s) -
Shin KyungChul,
Seo MinJu,
Ju JeongHun,
Oh DeokKun
Publication year - 2019
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1002/aocs.12219
Subject(s) - escherichia coli , recombinant dna , linoleic acid , dimethyl sulfoxide , oleic acid , chemistry , biochemistry , substrate (aquarium) , fatty acid , biology , organic chemistry , gene , ecology
Recombinant Escherichia coli expressing T879A variant 6,8‐linoleate diol synthase (LDS) from Penicillium oxalicum showed 2.1‐fold higher activity than recombinant E. coli expressing wild‐type 6,8‐LDS for the production of 6,8‐dihydroxy fatty acids (DiHFA) from linoleic acid. The optimal conditions for the production of 6,8‐DiHFA by recombinant E. coli expressing T879A variant 6,8‐LDS were pH 6.5, 35°C, 50 g L −1 cells, 10 g L −1 (35.7 mM) substrate, and 5% (v/v) dimethyl sulfoxide. Under these optimized conditions, 6.6 g L −1 (22.1 mM) 6,8‐dihydroxy‐9,12( Z , Z )‐octadecadienoic acid (DiHODE) and 7.1 g L −1 (22.6 mM) 6,8‐dihydroxy‐9( Z )‐octadecenoic acid (DiHOME) were produced from linoleic acid and oleic acid in 40 min, respectively. The volumetric productivities of 6,8‐DiHODE and 6,8‐DiHOME under these conditions were 9.9 and 10.7 mg L −1 h −1 , respectively. The volumetric productivities of 6,8‐DiHODE and 6,8‐DiHOME were the highest values among those of all reported regiospecific DiHODE and DiHOME, respectively. To the best of our knowledge, this is the first quantitative biotechnological production of 6,8‐DiHFA.