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Enhanced Synthesis of Fatty‐Acid Methyl Ester using Oil from Palm Oil Mill Effluents and Immobilized Palm Lipase
Author(s) -
Paichid Nisa,
Yunu Tewan,
Klomklao Sappasith,
Prasertsan Poonsuk,
Sangkharak Kanokphorn
Publication year - 2018
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1002/aocs.12141
Subject(s) - pome , lipase , methanol , fatty acid methyl ester , chemistry , palm oil , substrate (aquarium) , fatty acid , catalysis , pulp and paper industry , nuclear chemistry , organic chemistry , food science , chromatography , biodiesel , enzyme , biology , ecology , engineering
Oil from palm oil mill effluents (POME) was utilized as the substrate for fatty‐acid methyl ester (FAME) production using lipase from palm fruit immobilized to chitosan beads as a catalyst. The optimal reaction conditions consisted of a methanol‐to‐oil ratio of 6:1 mol mol −1 , 6 g immobilized lipase per 10 g of POME oil at an incubation temperature of 35°C, a mixing speed of 200 rpm (with a 5 cm orbital diameter), and a reaction time of 24 h. Under optimal conditions, the FAME content was 93.6% methanol‐free basis. In addition, immobilized lipase retained 90.5% after six subsequent reaction cycles (compared with the FAME content of the first batch). The properties of FAME were characterized and found to fulfill both Thailand and ASTM standards.