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A luminescence probe for c‐myc G‐quadruplex by a triphenylamine‐appended ruthenium complex
Author(s) -
Liu XueWen,
Liu NingYi,
Deng YuanQing,
Wang Shan,
Liu Ting,
Tang YuCai,
Chen YuanDao,
Lu JiLin
Publication year - 2021
Publication title -
applied organometallic chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 71
eISSN - 1099-0739
pISSN - 0268-2605
DOI - 10.1002/aoc.6143
Subject(s) - chemistry , triphenylamine , luminescence , ruthenium , g quadruplex , förster resonance energy transfer , steric effects , selectivity , affinities , ligand (biochemistry) , stereochemistry , fluorescence , dna , photochemistry , organic chemistry , receptor , biochemistry , physics , optoelectronics , quantum mechanics , catalysis
Due to the essential roles of G‐quadruplex in biological processes, the development of G‐quadruplex luminescent probe is becoming more and more important for further understanding of the functions of G‐quadruplex in biology. We herein reported that a ruthenium complex containing triphenylamine group exhibited “off–on” emission behavior after binding to G‐quadruplex in the presence of luminescence quencher [Fe (CN 6 )] 4− . The Ru‐Fe system consists of a mixture of [Ru (phen)2(TPAD)]2+, where TPAD = N‐4‐(1H‐imidazo [4,5‐f] [1,10]phenanthroline‐2‐yl phenyl)‐N‐phenyl‐benzenamine and [Fe (CN 6 )] 4− , which showed luminescent selectivity toward parallel G‐quadruplex (c‐myc). The detection limit was 159 nM for c‐myc. The ligand TPAD bearing nonplanar triphenylamine group and steric hindrance led to different DNA affinities toward various DNA structures. Luminescence experiments, CD spectra, G4‐FID, FRET (fluorescence resonance energy transfer) measurements, and molecular docking results indicated that different DNA affinities and binding modes of the complex toward various DNA structures result in the luminescence selectivity.