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Biofunctionalized silver and gold nanoparticles as potential curative agents
Author(s) -
Priya Velammal Subramanian,
Peter Amaladhas Thomas
Publication year - 2021
Publication title -
applied organometallic chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 71
eISSN - 1099-0739
pISSN - 0268-2605
DOI - 10.1002/aoc.6044
Subject(s) - chemistry , nuclear chemistry , colloidal gold , nanoparticle , silver nanoparticle , fourier transform infrared spectroscopy , transmission electron microscopy , quenching (fluorescence) , mtt assay , dpph , nanotechnology , organic chemistry , fluorescence , chemical engineering , antioxidant , in vitro , biochemistry , materials science , physics , quantum mechanics , engineering
In this study, the bark of an important medicinal plant, Indigofera aspalathoides is utilized as a bioreductant for the synthesis of silver nanoparticles (AgNPs) and gold nanoparticles (AuNPs). The formation of nanoparticles was monitored, and the reaction parameters were optimized by UV–Vis spectroscopy. The attachment of biocomponents as stabilizer was proved employing Fourier‐transform infrared (FT‐IR) studies. Through transmission electron microscopy (TEM), the morphology was found to be predominantly spherical and a mixture of triangle and hexagon in the case of AgNPs and AuNPs, respectively. The crystallite size of AgNPs and AuNPs was affirmed through X‐ray diffraction (XRD) studies using Sherrer formula as 22.03 and 47.70 nm, respectively. DPPH method was adopted to analyse the free‐radical quenching ability, and the AgNPs, AuNPs and extract showed inhibition of 76%, 89% and 59% at a concentration of 200 μg ml −1 , and the corresponding IC 50 values were 86.49, 55.20 and 149.19 μg ml −1 . The binding of nanoparticles to calf‐thymus DNA (CT‐DNA) was through groove and the high binding constants (8.49 × 10 6 M −1 and 2.34 × 10 7 M −1 for AgNPs and AuNPs) point out the potential of these nanoparticles as curative drugs. The MTT assay showed that AgNPs were 100% toxic, and the low IC 50 value suggests that this can be used in the medicinal field as a safe drug.