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Uptake and degradation of arsenobetaine by the microorganisms occurring in sediments
Author(s) -
Hanaoka Ken'ichi,
Uchida Kenji,
Tagawa Shoji,
Kaise Toshikazu
Publication year - 1995
Publication title -
applied organometallic chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 71
eISSN - 1099-0739
pISSN - 0268-2605
DOI - 10.1002/aoc.590090712
Subject(s) - arsenobetaine , chemistry , microorganism , incubation , chromatography , degradation (telecommunications) , environmental chemistry , arsenic , high performance liquid chromatography , mass spectrometry , biochemistry , organic chemistry , bacteria , inductively coupled plasma mass spectrometry , biology , telecommunications , computer science , genetics
We have reported the degradation of arsenobetaine [(CH 3 ) 3 As + CH 2 COO − ] to inorganic arsenic by microorganisms from various marine origins such as sediments. However, there was no information as to the fate of the ingested arsenobetaine within the body of the microorganisms before excretion. In this study, arsenobetaine and sediments were added to two culture media (1/5 Zobell 2216E and a solution of inorganic salts) and aerobically incubated at 25°C in the dark. Despite the degradation and complete disappearance of arsenobetaine from the filtrates of the incubation mixtures, the major arsenic compound from the microorganisms harvested from the mixtures was identified by HPLC as arsenobetaine throughout the incubation period. The presence of arsenobetaine was further confirmed by TLC and fast atom bombardment mass spectrometry (FAB MS). A minor arsenical also present in the incubated microorganisms, dimethylarsinic acid, was detected.