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Arsenobetaine as the major arsenic compound in the muscle of two species of freshwater fish
Author(s) -
Shiomi Kazuo,
Sugiyama Yasuo,
Shimakura Kuniyoshi,
Nagashima Yuji
Publication year - 1995
Publication title -
applied organometallic chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 71
eISSN - 1099-0739
pISSN - 0268-2605
DOI - 10.1002/aoc.590090203
Subject(s) - arsenobetaine , chemistry , freshwater fish , arsenic , fish <actinopterygii> , zoology , environmental chemistry , fishery , organic chemistry , inorganic arsenic , biology
The chemical form of arsenic contained in the muscle of certain freshwater fish was examined using cultured specimens of rainbow trout ( Salmo gairdneri ) and wild specimens of Japanese smelt ( Hypomesus nipponensis ). More than 95% of the total arsenic of both species was extracted with methanol and recovered in the water‐soluble fraction. The major arsenic compound of both species was purified by cation‐exchange chromatography on Dowex 50, gel filtration on Bio‐Gel P‐2 and HPLC on Asahipak GS‐220H. Behavior in the above purification procedure and analyses of the purified compounds by HPLC–ICP and TLC confirmed that the major arsenic compound of both species was arsenobetaine. Arsenobetaine found in cultured rainbow trout seems to be derived from the commercial assorted feed containing arsenobetaine as the major arsenical. On the other hand, the result with wild Japanese smelt suggested that arsenobetaine is a naturally occurring compound in the freshwater environment.