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Optimization of method for speciation of mercury(II), monomethylmercury cation, dimethylmercury and diethylmercury by hydride generation
Author(s) -
Weber James H.,
Puk Richard
Publication year - 1994
Publication title -
applied organometallic chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 71
eISSN - 1099-0739
pISSN - 0268-2605
DOI - 10.1002/aoc.590080723
Subject(s) - chemistry , mercury (programming language) , analyte , atomic absorption spectroscopy , hydride , quartz , analytical chemistry (journal) , chromatography , organic chemistry , metal , metallurgy , physics , materials science , quantum mechanics , computer science , programming language
This paper describes optimization details of an analytical method development of a hydride‐generation procedure for speciation of mercury(II), monomethylmercury cation, dimethylmercury, and diethylmercury using a heated quartz furnace with an atomic absorption spectrophotometer (AAS) as a detector. Typically a new analytical method is developed in steps such as the following: (1) determining analytes individually to confirm retention times and the lack of decomposition during the procedure; (2) comparing peak areas of all analytes as a function of quartz furnace temperature to optimize atomization for AAS detection; (3) conducting factorial experiments to determine which hydride‐generation reaction conditions are important in maximizing peak areas and which conditions interact with each other; (4) using the simplex optimization method to give final optimization of reaction conditions. These steps result in conditions that maximize optimized peak areas for analytes while minimizing experimental error.