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Altered protein synthesis in rat kidney cells exposed to semiconductor materials
Author(s) -
Aoki Yasunobu,
Lipsky Michael M.,
Fowler Bruce A.
Publication year - 1994
Publication title -
applied organometallic chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 71
eISSN - 1099-0739
pISSN - 0268-2605
DOI - 10.1002/aoc.590080316
Subject(s) - chemistry , arsenite , sodium arsenite , gallium , methionine , chloride , biochemistry , arsenic , indium , amino acid , organic chemistry
It is thought that the extensive industrial use of arsenic, gallium and indium, which have applications as the materials for III–V semiconductors, will increase human exposure to these compounds in the near future. We have undertaken the development of new biological indicators for assessing exposure to these elements. Element‐specific alterations in protein synthesis patterns were expected to occur following exposure to arsenic compounds. We examined alterations in protein synthesis in primary cultures of rat kidney proximal tubule epithelial cells by sodium arsenite, gallium chloride and indium chloride, utilizing two‐dimensional gel electrophoresis. After incubation with the chemicals for 20 h, newly synthesized proteins were labeled with [ 35 S]methionine. A protein with a molecular weight ( M r ) of 30 000 was markedly induced on exposure to 10 μ M arsenite or 300 μ M gallium chloride, and synthesis of proteins with M r values of 85 000, 71 000, 65 000, 51 000, 38 000 and 28 000 were also increased by exposure to arsenite and gallium chloride. No significant changes were observed upon exposure to indium. Some of these increased proteins could be heat‐shock proteins.