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Different acute effects of oral and intratracheal administration of disodium arsenate and gallium arsenide on heme synthesis in rats
Author(s) -
Kondo Masao,
Ichikawa Isamu
Publication year - 1994
Publication title -
applied organometallic chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 71
eISSN - 1099-0739
pISSN - 0268-2605
DOI - 10.1002/aoc.590080310
Subject(s) - chemistry , oral administration , arsenic , sodium arsenate , saline , pharmacology , arsenate , medicine , organic chemistry
The acute influences of arsenic compounds on the metabolism of porphyrins and heme in various organs of rats after oral or intratracheal administration of disodium arsenate (Na 2 HAsO 4 ) and gallium arsenide (GaAs) were examined and compared. For the oral administration experiments, 21 or 84 mg of Na 2 HAsO 4 , or 2 or 4 g of GaAs, per cm 3 saline per kg body weight of each animal was administered to Jcl: Wistar male rats and the organs were removed after exsanguination from the vein of the right axilla under anesthesia with ether, 16 h after administration. In the case of intratracheal administration, rats given 8.2 or 16.4 mg of Na 2 HAsO 4 , or 0.2 or 0.4 g GaAs per cm 3 saline per kg body weight were examined under the same experimental conditions as for the administration route. Increase in the body weight of rats was suppressed after intratracheal administration of the two arsenic compounds. In these rats the hematocrit value increased significantly. These changes were not shown by the orally administered rats. Elevation in δ‐aminolevulinate synthase (ALA‐S, EC 2.3.1.37) activity in erythroblasts by Na 2 HAsO 4 was much higher after intratracheal administration than after oral administration. Suppression in the activities of δ‐aminolevulinate dehydratase (ALA‐D, EC 4.2.1.24) and porphobilinogen deaminase (PBG‐D, EC 4.3.1.8) in peripheral erythrocytes by Na 2 HAsO 4 and GaAs were stronger by intratracheal administration than by the oral route. Influences of GaAs on the activity of PBG‐D in rat liver were shown to be more effective by oral administration than by the intratracheal route. Oral administration of Na 2 HAsO 4 and GaAs had a stronger suppression effect on the activities of ALA‐D and PBG‐D in rat kidney. It seems from these results that the different extents of the influence of arsenic compounds might depend on the routes of intake.