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Effects of tributyltin(IV) chloride exposure on early embryonic stages of Ciona intestinalis : In vivo and ultrastructural investigations
Author(s) -
Mansueto C,
Gianguzza M,
Dolcemascolo G,
Pellerito L
Publication year - 1993
Publication title -
applied organometallic chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 71
eISSN - 1099-0739
pISSN - 0268-2605
DOI - 10.1002/aoc.590070606
Subject(s) - chemistry , ciona intestinalis , tributyltin , ultrastructure , chloride , in vivo , incubation , cytoplasm , embryo , blastomere , organelle , biophysics , embryogenesis , microbiology and biotechnology , biochemistry , anatomy , biology , environmental chemistry , organic chemistry , gene
The effects of tributyltin(IV) chloride (TBT chloride) solutions on ascidian embryos of Ciona intestinalis have been tested at different stages of development. It has been observed, in vivo , that TBT chooride inhibited cleavage of fertilized eggs and of embryo blastomeres giving rise to cellular masses that are not delimited by plasma membrane. Electron‐dense precipitates of TBT chloride, probably as inorganic tin, have been observed by transmission electron microscopy in the egg cytoplasm of cellular masses. The same type of precipitate was present also inside the mitochondria, whose structure appeared to be highly modified. These ultrastructure aspects are indicative of a degenerative process of the embroys after incubation in TBT chloride.