Premium
Lipophilic arsenic compound(s) in the liver of a tiger shark (Galeocerdo cuvier)
Author(s) -
Miyajima Mariko,
Hamada Noriaki,
Yoshimura Etsuro,
Okubo Akira,
Yamazaki Sunao,
Toda Shozo
Publication year - 1988
Publication title -
applied organometallic chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 71
eISSN - 1099-0739
pISSN - 0268-2605
DOI - 10.1002/aoc.590020416
Subject(s) - chemistry , chloroform , methanol , chromatography , arsenic , silica gel , acetic acid , column chromatography , thin layer chromatography , organic chemistry
Homogenized aliquots (100 g) of the liver (8.4 kg, 5 m̈g As g −1 ) of a tiger shark (Galeocerdo cuvier) were extracted with chloroform/methanol, and the extracts purified by countercurrent extraction (hexane/87% ethanol), silica gel column chromatography (chloroform/methanol mixtures as mobile phases), and silica gel thin‐layer chromatography (chloroform/methanol/acetic acid). The purified samples (24 mg arsenic g −1 ) gave no 31 P NMR signal, but gave 1 H and 13 C NMR signals with similarities to those of dipalmitoylphosphatidic acid and salad on and also signals indicative of the presence of methylated arsenic compounds. The sample could contain a diacyl glyceride with a methylated arsenic group.