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Fluorescence microscopy‐based analysis of apoptosis induced by platinum nanoparticles against breast cancer cells
Author(s) -
Puja Patel,
Vinita Nadar Manimaran,
Devan Umapathy,
Velangani Antony Joseph,
Srinivasan Pappu,
Yuvakkumar Rathinam,
Arul Prakash Pitchan,
Kumar Ponnuchamy
Publication year - 2020
Publication title -
applied organometallic chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 71
eISSN - 1099-0739
pISSN - 0268-2605
DOI - 10.1002/aoc.5740
Subject(s) - platinum , chemistry , platinum nanoparticles , nanomaterials , nanoparticle , nanotechnology , fluorescence microscope , cancer cell , dispersity , biophysics , fluorescence , materials science , cancer , organic chemistry , catalysis , physics , quantum mechanics , biology , medicine
In recent years, capping molecules onto the surface of nanomaterials has become an interesting field of research. This idea facilitates the biological applications of nanomaterials with a modified surface. Keeping this in mind, the present study addresses the development of polymeric platinum nanoparticles using polyvinyl pyrrolidone (PVP). High‐throughput characterization indicates that polymeric platinum nanoparticles have an excellent surface morphology and good dispersity in aqueous solution. More specifically, high resolution‐transmission electron microscopy studies showed that the polymeric platinum nanoparticles were spherical and measured 2–10 nm. Furthermore, the polymeric platinum nanoparticles were evaluated for anticancer properties against human MCF‐7 breast cancer cell lines. The results show that polymeric platinum nanoparticles inhibited the growth of cancer cells in a dose‐dependent manner with a half‐maximum inhibitory concentration of 96.36 μg ml −1 . In addition, fluorescence‐based staining methods confirmed an inquest in the pattern of cell death inferring late apoptotic bodies, nuclear fragmentation, mitochondrial membrane potential and generation of reactive oxygen species. The overall findings suggest that the polymeric platinum nanoparticles confer anticancer activity and may be suitable chemotherapeutic agents in the future. Finally, the results from this study can be extended to other types of cancer as well.