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Biosynthesis and characterization of Dillenia indica ‐mediated silver nanoparticles and their biological activity
Author(s) -
Nayak Sreenivasa,
Bhat Meghashyama P.,
Udayashankar A.C.,
Lakshmeesha T.R.,
Geetha Nagaraja,
Jogaiah Sudisha
Publication year - 2020
Publication title -
applied organometallic chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 71
eISSN - 1099-0739
pISSN - 0268-2605
DOI - 10.1002/aoc.5567
Subject(s) - zeta potential , silver nanoparticle , chemistry , nuclear chemistry , fourier transform infrared spectroscopy , bacteria , enterococcus faecalis , antibacterial activity , nanoparticle , scanning electron microscope , escherichia coli , nanotechnology , biochemistry , chemical engineering , materials science , biology , engineering , composite material , genetics , gene
Dillenia indica L. is a traditional medicinal plant well known for its ability to cure various human diseases. In the current study, silver nanoparticles have been synthesized by simple and eco‐friendly method using Dillenia indica extract. The green synthesized nanoparticles were characterized by Fourier transform infrared (FTIR), UV–visible spectroscopy, Atomic force microscopy (AFM), High‐resolution transmission electron microscopy (HR‐TEM), Zeta Potential and Size Distribution. UV–visible and FTIR spectra, AFM, HR‐TEM and Zeta Potential readings and size distribution conformed that the synthesized silver particles were in the size of nano. The green synthesized silver nanoparticles were subjected for antibacterial activity against Gram‐positive bacteria Enterococcus faecalis and Gram‐negative bacteria Escherichia coli by agar well diffusion method. The synthesized AgNPs exhibited significant inhibition of 27 and 16 mm against the test bacteria at 0.25 mg/ml. Further the antibacterial activity was confirmed by live and dead cell assay by fluorescence microscopy and morphological changes of bacteria were studied by Scanning electron microscope (SEM). The study recommends that the synthesized silver nanoparticles using Dillenia indica extract have potential application in inhibition of bacteria owing to their potent antibacterial activity.