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An aminoquinoline based fluorescent probe for sequential detection of Znic (II) and inorganic phosphate and application in living cell imaging
Author(s) -
Fu Jiaxin,
Chang Yongxin,
Li Bai,
Mei Huihui,
Xu Kuoxi
Publication year - 2019
Publication title -
applied organometallic chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 71
eISSN - 1099-0739
pISSN - 0268-2605
DOI - 10.1002/aoc.5162
Subject(s) - chemistry , fluorescence , salicylaldehyde , titration , detection limit , hydroxymethyl , phenol , phosphate , buffer solution , selectivity , nuclear chemistry , schiff base , inorganic chemistry , chromatography , catalysis , stereochemistry , organic chemistry , physics , quantum mechanics
A novel fluorescent probe 5‐(diethylamino)‐2‐(((2‐(hydroxymethyl)quinolin‐8‐yl)imino)methyl)phenol ( QS) was synthesized by condensation reaction of 8‐aminoquinoline derivative and 4‐(diethylamino)salicylaldehyde. It was found that the probe QS was capable of high selectivity and sensitivity about specific color and fluorescence changes towards Zn 2+ ion in EtOH‐H 2 O ( v /v = 4/1, 0.01 M, Tris–HCl buffer, pH = 7.30) solution. The interaction of QS with Zn 2+ ion illustrated a “turn‐on” fluorescence response at 550 nm (λ ex : 458 nm), moreover, after the subsequent addition of inorganic phosphate (Pi) into the solution above, a “turn‐off” fluorescence response was observed. The sensing ability of the probe QS towards Zn 2+ was confirmed by fluorescence titration, UV–Vis titration and HRMS analysis. Besides, the intracellular sensing behavior of QS with Zn 2+ and Pi were captured in living PC12 cells. The limit of detection (LOD) for Zn 2+ and Pi sensing was found to be 0.03 μM and 0.08 μM, respectively.