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Synthesis, crystal structure, DNA/bovine serum albumin binding and antitumor activity of two transition metal complexes with 4‐acylpyrazolone derivative
Author(s) -
Zhang YanPing,
Li Yue,
Xu GuanCheng,
Li JinYu,
Luo HuaYing,
Li JinYao,
Zhang Li,
Jia DianZeng
Publication year - 2019
Publication title -
applied organometallic chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 71
eISSN - 1099-0739
pISSN - 0268-2605
DOI - 10.1002/aoc.4668
Subject(s) - chemistry , hela , bovine serum albumin , intercalation (chemistry) , quenching (fluorescence) , dna , binding constant , crystal structure , fluorescence , crystallography , stereochemistry , binding site , cell , biochemistry , inorganic chemistry , physics , quantum mechanics
Two new complexes, namely [Cu 6 L 6 ] ( 1 ) and [Zn(HL) 2 ] ( 2 ) (H 2 L = N ‐(1‐phenyl‐3‐methyl‐4‐propenylidene‐5‐pyrazolone)‐2‐furancarboxylic acid hydrazide), have been synthesized and characterized. Single crystal X‐ray analysis indicates that complex 1 has a hexanuclear structure and complex 2 exhibits a mononuclear structure. The DNA/bovine serum albumin (BSA) binding properties of complexes 1 and 2 were investigated by absorption spectroscopy and fluorescence quenching. Both complexes could effectively intercalate to DNA with calculated quenching constants of 2.6 × 10 5 and 1.25 × 10 5 M −1 , respectively. The quenching mechanism of the intrinsic fluorescence of BSA by the complexes was found to be a static one. The cytotoxicities of 1 and 2 were investigated in two human tumor cell lines, human esophageal cancer cells (Eca‐109) and cervical cancer cells (HeLa). Complex 1 exhibits higher antitumor activity than 2 . Furthermore, 1 can inhibit HeLa cells by inducing apoptosis and G0/G1 phase cell cycle arrest. All results demonstrate that 1 and 2 both have DNA/BSA binding capacity and antitumor activity.