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Biosynthesis of silver nanoparticles using oak leaf extract and their application for electrochemical sensing of hydrogen peroxide
Author(s) -
Hemmati Saba,
Baghayeri Mehdi,
Kazemi Sanaz,
Veisi Hojat
Publication year - 2018
Publication title -
applied organometallic chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 71
eISSN - 1099-0739
pISSN - 0268-2605
DOI - 10.1002/aoc.4537
Subject(s) - cyclic voltammetry , hydrogen peroxide , chemistry , dielectric spectroscopy , scanning electron microscope , electrochemistry , nuclear chemistry , silver nanoparticle , nanocomposite , biosensor , nanoparticle , glassy carbon , transmission electron microscopy , electrode , nanotechnology , materials science , organic chemistry , biochemistry , composite material
A green method is proposed for synthesizing silver nanoparticles using oak leaf extract as a reductant, capper and stabilizing agent. The characterization of biosynthesized Ag@oak leaf extract (Ag@OLE) was performed using scanning electron microscopy–energy‐dispersive X‐ray analysis, X‐ray diffraction, transmission electron microscopy and UV–visible spectroscopy. The Ag@OLE nanocomposite was applied for immobilizing haemoglobin (Hb) on the surface of glassy carbon electrode (GCE) and its application as a hydrogen peroxide biosensor of was studied. Electrochemical properties of the biosensor were studied using cyclic voltammetry and electrochemical impedance spectroscopy. Direct electrical communication between the redox centre of Hb and GCE was created via conducting network of Hb–Ag@OLE.