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Application of novel copper organic material for facile microextraction of sodium valproate from human plasma samples: Experimental design optimization and isotherm study
Author(s) -
Bahrani Sonia,
Ghaedi Mehrorang,
Taghipour Tahere,
Mansoorkhani Mohammad Javad Khoshnood,
Bagheri Ahmad Reza,
Ostovan Abbas
Publication year - 2018
Publication title -
applied organometallic chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 71
eISSN - 1099-0739
pISSN - 0268-2605
DOI - 10.1002/aoc.3960
Subject(s) - chemistry , detection limit , central composite design , freundlich equation , adsorption , chromatography , sorbent , analytical chemistry (journal) , scanning electron microscope , fourier transform infrared spectroscopy , inductively coupled plasma , high performance liquid chromatography , selectivity , response surface methodology , plasma , chemical engineering , organic chemistry , materials science , engineering , composite material , catalysis , physics , quantum mechanics
Well‐designed metal organic materials (MOMs) were synthesized and applied for pre‐concentration and determination of sodium valproate (Na‐VP) from biological samples, bound to the copper complex of 1,4‐phenylenedioxydiacetic acid under mild conditions. The channels of this sorbent provide high efficiency and also selectivity. The MOMs were structurally characterized using Fourier transform infrared and energy‐dispersive X‐ray spectroscopies, scanning electron microscopy and X‐ray diffraction, and they were found to have suitable features for quantification of Na‐VP using HPLC coupled with UV detection at λ = 215 nm. Moreover, the rate of adsorption is improved by ultrasonic power and the experimental data are best fitted according to Freundlich adsorption isotherm. According to the central composite design, the best experimental conditions are 280.0 μl, 3.0 min and 17.0 mg for volume of eluent, sonication time and sorbent mass respectively. Calibration plots show linear responses towards Na‐VP concentrations (0.4–18.0 μg ml −1 ), satisfactory limit of detection (0.06 μg ml −1 , S/ N = 3) and reasonable enrichment factor (70.58). The coefficient of variation values of both inter‐ and intra‐day analyses were less than 4.0%, indicating a candidate method for the determination of Na‐VP in human plasma with reasonable recovery and efficiency.