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Preparation, characterization and biological evaluation of two chiral binuclear copper(II) complexes
Author(s) -
Suo JingJing,
Ma ZhongYing,
Xu JingYuan,
Tian JinLei,
Liu Xin
Publication year - 2018
Publication title -
applied organometallic chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 71
eISSN - 1099-0739
pISSN - 0268-2605
DOI - 10.1002/aoc.3911
Subject(s) - chemistry , intercalation (chemistry) , dna , cytotoxicity , copper , a549 cell , comet assay , stereochemistry , in vitro , cleavage (geology) , dna damage , crystallography , biochemistry , organic chemistry , geotechnical engineering , fracture (geology) , engineering
Two chiral Cu(II) complexes of [Cu 2 ( R ‐L) 2 ](PF 6 ) 2 ·2C 2 H 5 OH ( 1 ) and [Cu 2 ( S ‐L) 2 ](PF 6 ) 2 ·2C 2 H 5 OH ( 2 ) (HL = 2‐(Bis(quinolin‐2‐ylmethyl)amino)‐1‐propanol) were designed and synthesized to serve as chemical nucleases and anticancer drugs. X‐ray crystallography revealed that two complexes contain chiral binuclear cations and PF 6 − anions. The interaction of two complexes with CT‐DNA was researched via various spectroscopic techniques and viscosity measurement, indicating that the complexes were bound to CT‐DNA by a classical intercalation binding mode. In addition, the two complexes exhibited remarkable DNA cleavage activity with an optimal dosage of 10 μM in the absence of any exogenous oxidant agent. Both of the complexes showed excellent in vitro cytotoxicity on A549 cell lines with IC 50 values in the low micromolar range. Moreover, complex 2 could damage DNA of A549 cells into fragmentation and then induced cell apoptosis in a dose‐dependent manner, which was demonstrated by comet assay and Hoechst 33342 staining experiment. Further research showed that complex 2 could also induce G 2 and S phase cell cycle arrest.