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Ga(III) complex with morin for kidney cancer cell labelling
Author(s) -
Sentkowska Aleksandra,
Kilian Krzysztof,
Kopeć Maciej,
Pyrzyńska Krystyna,
Cheda Łukasz
Publication year - 2017
Publication title -
applied organometallic chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 71
eISSN - 1099-0739
pISSN - 0268-2605
DOI - 10.1002/aoc.3882
Subject(s) - morin , chemistry , metal , molecule , stoichiometry , flavonoid , atom (system on chip) , stereochemistry , organic chemistry , medicine , pathology , antioxidant , computer science , embedded system
The formation of a complex between Ga(III) and morin (3,5,7,2′,4′‐pentahydroxyflavone) was studied. UV–visible, infrared and mass spectroscopies were used to characterize the complex. The stoichiometric ratio for the reaction between metal ion and flavonoid was determined using the methods of Yoe–Jones and Job, which confirmed that a 1:1 Ga–morin complex was formed (estimated binding constant = 2.31 × 10 4 l mol −1 ). It was found that the coordination to Ga(III) occurs through the carbonyl oxygen atom and the 3‐OH group of the morin molecule. According to developed conditions, complexation reaction with 68 Ga was performed and the complex was used to label kidney cancer cells (CAKI‐1, CAKI‐2, ACHN and 786‐O). The knowledge gained from this study should be useful for the development of new radiopharmaceuticals for diagnostic purposes containing 68 Ga.