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Biological evaluation of organometallic palladium(II) complexes containing 4‐hydroxybenzoic acid (3‐ethoxy‐2‐hydroxybenzylidene)hydrazide: Synthesis, structure, DNA/protein binding, antioxidant activity and cytotoxicity
Author(s) -
Ayyannan Ganesan,
Veerasamy Perumal,
Mohanraj Maruthachalam,
Raja Gunasekaran,
Manimaran Arumugam,
Velusamy Marappan,
Bhuvanesh Nanjan,
Nandhakumar Raju,
Jayabalakrishnan Chinnasamy
Publication year - 2017
Publication title -
applied organometallic chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 71
eISSN - 1099-0739
pISSN - 0268-2605
DOI - 10.1002/aoc.3599
Subject(s) - chemistry , palladium , bovine serum albumin , ligand (biochemistry) , hela , hydrazide , absorption spectroscopy , stereochemistry , nuclear chemistry , organic chemistry , in vitro , biochemistry , physics , receptor , quantum mechanics , catalysis
New palladium(II) complexes, [Pd(PPh 3 )L] ( 2 ) and [Pd(AsPh 3 )L] ( 3 ), were synthesized using 4‐hydroxybenzoic acid (3‐ethoxy‐2‐hydroxybenzylidene)hydrazide ( 1 ) ligand (H 2 L), and characterized using various physicochemical techniques. The molecular structures of 2 and 3 were determined using single‐crystal X‐ray diffraction, which reveals a square planar geometry around the palladium(II) metal ion. In vitro DNA binding studies were conducted using UV–visible absorption spectroscopy, emission spectroscopy, cyclic voltammetry and viscosity measurements, which suggest that the metal complexes act as efficient DNA binders. The interaction of ligand H 2 L and complexes 2 and 3 with bovine serum albumin (BSA) was investigated using UV–visible and fluorescence spectroscopies. Absorption and emission spectral studies indicate that complexes 2 and 3 interact with BSA protein more strongly than the parent ligand. The free radical scavenging potential of all the synthesised compounds ( 1 – 3 ) was also investigated under in vitro conditions. In addition, the in vitro cytotoxicity of the complexes to tumour cells lines (HeLa and MCF‐7) was examined using the MTT assay method.