Premium
Biological screening and DNA nuclease activity of transition metal complexes of N 2 O 2 type of Knoevenagel condensate Schiff base
Author(s) -
Pandiyan Rajakkani Paul,
Raman Natarajan
Publication year - 2016
Publication title -
applied organometallic chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 71
eISSN - 1099-0739
pISSN - 0268-2605
DOI - 10.1002/aoc.3466
Subject(s) - chemistry , schiff base , intercalation (chemistry) , knoevenagel condensation , transition metal , carbon 13 nmr , stereochemistry , metal , crystallography , nuclear chemistry , inorganic chemistry , organic chemistry , catalysis
A novel tetradentate N 2 O 2 type of Knoevenagel condensate Schiff base, synthesized from 4‐amino‐2,3‐dimethyl‐1‐phenyl‐3‐pyrazolin‐5‐one (4‐aminoantipyrine) and 3‐(cinnamyl)‐pentane‐2,4‐dione, forms stable complexes with transition metal ions such as Cu(II), Co(II), Ni(II) and Zn(II). The structural features were derived from elemental analysis, molar conductance measurements, infrared, UV–visible, 1 H NMR, 13 C NMR, mass and electron paramagnetic resonance spectroscopies. These complexes show high conductance values, supporting their electrolytic nature. Spectroscopic and other analytical data of the complexes suggest square planar geometry. In vitro calf thymus DNA binding studies were performed by employing UV–visible absorption spectroscopy, viscometry and cyclic voltammetry. These techniques indicate that all the metal complexes bind to DNA via intercalation mode. Antimicrobial screening of the synthesized ligand and complexes was conducted against Gram‐positive bacteria, Gram‐negative bacteria and fungi. These complexes exhibit higher antimicrobial activities than the free Schiff base, as investigated using the minimum inhibitory concentration method. Gel electrophoresis reveals that these complexes also promote the cleavage of pUC18 plasmid DNA in the presence of activators. Copyright © 2016 John Wiley & Sons, Ltd.