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Cytotoxicological aspects of the organic arsenic compound arsenobetaine in marine animals
Author(s) -
Kojima Chikara,
Sakurai Teruaki,
Ochiai Masayuki,
Kumata Hidetoshi,
Qu Wei,
Waalkes Michael P.,
Fujiwara Kitao
Publication year - 2002
Publication title -
applied organometallic chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 71
eISSN - 1099-0739
pISSN - 0268-2605
DOI - 10.1002/aoc.328
Subject(s) - chemistry , cytotoxicity , arsenobetaine , glutathione , biochemistry , secretion , nitric oxide , in vitro , macrophage , arsenite , microbiology and biotechnology , arsenic , enzyme , organic chemistry , biology
In this study, we investigated the in vitro cytotoxicity of arsenobetaine [AsBe; trimethyl (carboxymethyl) arsonium zwitterion], which is a major organic arsenic compound in marine animals, to various mammalian cells, such as mouse macrophage RAW264.7 cells, rat liver TRL1215 cells and human skin TIG‐112 cells, using a synthetic pure material. As a result, we demonstrated that the cytotoxicity of AsBe is very weak even at concentrations over 20 mmol dm −3 in all these cells. Also, AsBe did not affect various functions of the murine macrophage RAW264.7 cells, viz interleukin‐1α production, cellular lysosomal enzyme (acid phosphatase) activity and nitric oxide (NO 2 − ) secretion. AsBe showed a weak effect on the reduced glutathione (GSH) levels in these cells, and it slightly reduced the cellular GSH levels for a while. These data suggest that AsBe shows no cytotoxicity but has some effects on mammalian cells. Copyright © 2002 John Wiley & Sons, Ltd.