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Effects of the fractionated components of the seaweed Hizikia fusiforme Okam. on arsenic metabolism in rats administered a large dose of arsenate
Author(s) -
Katayama Masayuki,
Kouya Yukie,
Furusawa Chiduru,
Sakiyama Chie,
Kaise Toshikazu,
SugawaKatayama Yohko
Publication year - 2002
Publication title -
applied organometallic chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 71
eISSN - 1099-0739
pISSN - 0268-2605
DOI - 10.1002/aoc.321
Subject(s) - chemistry , arsenic , arsenate , urine , chromatography , metabolism , biochemistry , organic chemistry
To investigate factors in the seaweed Hijiki affecting arsenic metabolism in rats, we extracted pulverized Hijiki samples with hot 0.1 M NaOH, and neutralized the extract with HCl. After centrifugation at 10 000 rpm for 30 min, the supernatants and the residues were lyophilized, mixed with cellulose, and added to the standard diet for rats. The rats were divided into three groups as follows: (1) group C , fed the standard diet, (2) group H‐sup , fed a diet containing the extracted soluble fraction, and (3) group H‐rsd , fed a diet containing the residues. After feeding the diets for 12 days, the respective groups were divided into two sub‐groups; one of them was administered a large oral single dose (40% of the LD 50 ) of an aqueous solution of sodium arsenate, Na 2 AsHO 4 ·7H 2 O, 24 h before sacrifice. Samples of urine were gathered before and after the administration of arsenate. Arsenic compounds in the urine and serum were analyzed by high‐performance liquid chromatography–inductively coupled plasma mass spectrometry system. The patterns of the arsenic compounds suggested that some Hijiki components accelerated arsenic metabolism, e.g. methylation, in rats. Copyright © 2002 John Wiley & Sons, Ltd.