Premium
Copper(II) complexes of acylhydrazones: synthesis, characterization and DNA interaction
Author(s) -
Gökçe Cansu,
Gup Ramazan
Publication year - 2013
Publication title -
applied organometallic chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 71
eISSN - 1099-0739
pISSN - 0268-2605
DOI - 10.1002/aoc.2955
Subject(s) - chemistry , copper , agarose gel electrophoresis , singlet oxygen , dna , cleavage (geology) , titration , hydrogen peroxide , infrared spectroscopy , gel electrophoresis , stereochemistry , polymer chemistry , crystallography , inorganic chemistry , oxygen , organic chemistry , biochemistry , geotechnical engineering , fracture (geology) , engineering
Two new acylhydrazone copper(II) complexes of 4‐hydroxy‐ N ′‐[(1 E )‐1‐(4‐methylphenyl)ethylidene]benzohydrazide (HL 1 ) and 4 ethyl [4‐({(2 E )‐2‐[1‐(4‐methylphenyl)ethylidene]hydrazinyl}carbonyl)phenoxy]acetate (HL 2 ) have been synthesized and characterized. The structures of both acylhydrazone and copper(II) complexes were identified by elemental analysis, infrared spectra, UV–visible electronic absorption spectra, magnetic susceptibility measurements, TGA and powder X‐ray diffraction. DNA binding and DNA cleavage activities of the synthesized copper complexes were examined by using UV‐visible titration and agarose gel electrophoresis, respectively. The effect of complex concentration on the DNA cleavage reactions in the absence and presence of H 2 O 2 was also investigated. The results indicate that all the complexes bind slightly to calf thymus DNA and cleavage pBR322 DNA. The mechanistic studies demonstrate that a hydrogen peroxide‐derived species and singlet oxygen ( 1 O 2 ) are the active oxidative species for DNA cleavage. Copyright © 2013 John Wiley & Sons, Ltd.