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Effect of different organotin compounds on DNA of gilthead sea bream ( Sparus aurata ) erythrocytes assessed by the comet assay
Author(s) -
Gabbianelli Rosita,
Villarini Milena,
Falcioni Giancarlo,
Lupidi Giulio
Publication year - 2002
Publication title -
applied organometallic chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 71
eISSN - 1099-0739
pISSN - 0268-2605
DOI - 10.1002/aoc.275
Subject(s) - tributyltin , chemistry , biocide , biofouling , comet assay , dna damage , chloride , genotoxicity , incubation , dna , environmental chemistry , biochemistry , toxicity , membrane , organic chemistry
The ‘comet’ assay appears to be a promising tool for estimating DNA damage at the single cell level. We used this test to evaluate the effect of organotin compounds on sea bream nucleated erythrocytes. The tributyltin chloride (TBTC), dibutyltin chloride (DBTC) and monobutyltin chloride (MBTC) employed in this study show different genotoxicities. TBTC and DBTC have pronounced effects on tail length, tail intensity and tail moment, though TBTC is more efficient in producing DNA damage. MBTC leads to a fast genotoxic effect that does not change with the incubation time. The data obtained are important for the analysis of the environmental risks produced by organotin compounds used as antifouling agents in marine paints and as biocides in agriculture. Copyright © 2002 John Wiley & Sons, Ltd.