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DNA interaction, free radical scavenging and in‐vitro antibacterial activity of drug‐based copper(II) complexes
Author(s) -
Patel Mohan N.,
Dosi Promise A.,
Bhatt Bhupesh S.
Publication year - 2011
Publication title -
applied organometallic chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 71
eISSN - 1099-0739
pISSN - 0268-2605
DOI - 10.1002/aoc.1817
Subject(s) - chemistry , agarose gel electrophoresis , copper , dna , titration , puc19 , in vitro , superoxide dismutase , antibacterial activity , nuclear chemistry , stereochemistry , inorganic chemistry , bacteria , organic chemistry , biochemistry , antioxidant , biology , plasmid , genetics
The Cu(II) complexes of type [Cu(cpf)(A n )Cl] (A n = terpyridines, cpf = ciprofloxacin) were synthesized and characterized using IR, mass and reflectance spectra. The free ligands and their complexes were evaluated for their in‐vitro antimicrobial activity against a panel of Gram‐positive and Gram‐negative bacteria. The complexes exhibit better or equal inhibition in comparison to free fluoroquinolones. Binding interactions of the complexes with calf thymus (CT DNA) were investigated by absorption titration, viscosity studies and DNA melting temperature experiment. The cleavage reaction on pUC19 DNA was monitored by agarose gel electrophoresis. The lower concentration of the complexes was catalysed the dismutation of superoxide radical at biological pH, which indicates that the complexes can act as a possible model for superoxide dismutase. Copyright © 2011 John Wiley & Sons, Ltd.