Premium
Cyclometalated iridium(III) complex of 6‐hydroxydipyrido[3,2‐ a :2′,3′‐ c ]phenazine: synthesis, and acid–base and avid DNA binding properties
Author(s) -
Chen YanMin,
Zhang AnGuo,
Wang KeZhi
Publication year - 2011
Publication title -
applied organometallic chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 71
eISSN - 1099-0739
pISSN - 0268-2605
DOI - 10.1002/aoc.1796
Subject(s) - chemistry , phenazine , binding constant , ethidium bromide , dna , titration , intercalation (chemistry) , density functional theory , crystallography , absorption spectroscopy , stereochemistry , binding site , inorganic chemistry , computational chemistry , organic chemistry , biochemistry , physics , quantum mechanics
A new cyclometalated Ir(III) complex [Ir(ppy) 2 (hdppz)]PF 6 (Hppy = 2‐phenylpyridine and hdppz = 6‐hydroxydipyrido[3,2‐ a :2′,3′‐c]phenazine) was synthesized and characterized. The pH effects on the UV–vis absorption spectra were studied and ground‐state acid ionization constant p K a values of the complex were derived. The calf thymus DNA (ct‐DNA) binding properties of the complex were investigated with UV‐vis absorption spectrophotometric titrations, DNA competitive binding with ethidium bromide, DNA melting experiments, viscosity measurements and density functional theory (DFT) calculations. The complex was demonstrated to act as a ct‐DNA intercalator with a large DNA binding constant value of (6.06 ± 0.32) × 10 6 M −1 in 50 m M NaCl. The avid DNA binding affinity observed was rationalized by the DFT calculations. Copyright © 2011 John Wiley & Sons, Ltd.