Premium
Antibacterial, SOD mimic and nuclease activities of copper(II) complexes containing ofloxacin and neutral bidentate ligands
Author(s) -
Patel Mohan N.,
Parmar Pradhuman A.,
Gandhi Deepen S.
Publication year - 2011
Publication title -
applied organometallic chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 71
eISSN - 1099-0739
pISSN - 0268-2605
DOI - 10.1002/aoc.1684
Subject(s) - chemistry , phenanthroline , copper , nuclease , nuclear chemistry , ofloxacin , stereochemistry , antibacterial activity , denticity , metal , puc19 , titration , ligand (biochemistry) , medicinal chemistry , dna , inorganic chemistry , organic chemistry , biochemistry , antibiotics , receptor , bacteria , ciprofloxacin , biology , plasmid , genetics
Drug‐based mixed‐ligand copper(II) complexes of type [Cu(OFL)(A n )Cl]·5H 2 O (OFL = ofloxacin, A 1 = pyridine‐2‐carbaldehyde, A 2 = 2,2′‐bipyridylamine, A 3 = thiophene‐2‐carbaldehyde, A 4 = 2,9‐dimethyl‐1,10‐phenanthroline, A 5 = 2,9‐dimethyl‐4,7‐diphenyl‐1,10‐phenanthroline, A 6 = 4,5‐diazafluoren‐9‐one, A 7 = 1,10‐phenanthroline‐5,6‐dione and A 8 = 5‐nitro‐1,10‐phenanthroline) were synthesized and characterized. Spectral investigations of complexes revealed square pyramidal geometry. Viscosity measurement and absorption titration were employed to determine the mode of binding of complexes with DNA. DNA cleavage study showed better cleaving ability of the complexes compared with metal salt and standard drug by conversion of a supercoiled form of pUC19 DNA to linear via circular. From the SOD mimic study, concentration of complexes ranging from 0.415 to 1.305 µ M is enough to inhibit the reduction rate of NBT by 50% (IC 50 ) in the NADH‐PMS system. Antibacterial activity was assayed against selective Gram‐negative and Gram‐positive microorganisms using the doubling dilution technique. Copyright © 2010 John Wiley & Sons, Ltd.