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Determination of degradation compounds derived from Lewisite by high performance liquid chromatography/inductively coupled plasma‐mass spectrometry
Author(s) -
Kinoshita Kenji,
Shikino Ozamu,
Seto Yasuo,
Kaise Toshikazu
Publication year - 2006
Publication title -
applied organometallic chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 71
eISSN - 1099-0739
pISSN - 0268-2605
DOI - 10.1002/aoc.1104
Subject(s) - chemistry , chromatography , formic acid , inductively coupled plasma mass spectrometry , high performance liquid chromatography , mass spectrometry , arsenic , acetonitrile , metabolite , detection limit , inductively coupled plasma , organic chemistry , plasma , biochemistry , physics , quantum mechanics
A method for determination of 2‐chlorovinylarsenous acid (CVAA) and 2‐chlorovinylarsonic acid (CVAOA), which are degradation compounds of the chemical warfare agent Lewisite, was examined by high‐performance liquid chromatography/inductively coupled plasma‐mass spectrometry (HPLC/ICP‐MS). Inertsil C 8 was suitable as the column and the mobile phase was consisted of 0.1% formic acid‐acetonitrile (80:20). These compounds were detected sensitively in a short time and separated from inorganic arsenicals and diphenylarsinic acid (DPAA) and phenylarsonic acid (PAA), which are degradation compounds of diphenylchloroarsine and phenyldichloroarsine, respectively. The detection limits of CVAA and CVAOA were 0.2 and 0.1 ngAs/ml, respectively. In addition, a dynamic reaction cell and oxygen as the reaction gas were applied, and then arsenic was detected as AsO + ( m / z 91) in order to prevent interference by ArCl + ( m / z 75). This method was applied to the analysis of urine obtained from a CVAA‐administered mouse and CVAOA was detected as the main metabolite. Thus, the speciation analysis of arsenic compounds derived from chemical warfare agents was achieved by HPLC/ICP‐MS. Copyright © 2006 John Wiley & Sons, Ltd.