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Cover Feature: A Syringe‐Based DNAzyme Sensor for Bacterial Detection (2/2021)
Author(s) -
Esmaeili Samani Sahar,
McConnell Erin M.,
Chang Dingran,
Rothenbroker Meghan,
Filipe Carlos D. M.,
Li Yingfu
Publication year - 2021
Publication title -
analysis and sensing
Language(s) - English
Resource type - Journals
ISSN - 2629-2742
DOI - 10.1002/anse.202100013
Subject(s) - deoxyribozyme , fluorescence , chemistry , chromatography , detection limit , physics , optics
The Cover Feature shows how a simple sensor comprising a syringe and a column containing agarose beads immobilized with an RNA‐cleaving DNAzyme specific for a bacterium of interest is set up to achieve bacterial detection by filtration. The DNAzyme is fluorescently labelled so that the bacterium‐induced cleavage event produces a fluorescent cleavage product that can be pushed out with the syringe onto a nitrocellulose paper sensor, which can then be detected using a handheld fluorescence device. More information can be found in the Communication by C. D. M. Filipe, Y. Li, and co‐workers.