Open AccessNovel technical developments in mass spectrometry imaging in 2020: A mini review
Author(s) -
Dilmetz Brooke A.,
Lee YeaRin,
Condina Mark R.,
Briggs Matthew,
Young Clifford,
Desire Christopher T.,
KlinglerHoffmann Manuela,
Hoffmann Peter
Publication year - 2021
Publication title -
analytical science advances
Language(s) - English
Resource type - Journals
ISSN - 2628-5452
DOI - 10.1002/ansa.202000176
Subject(s) - mass spectrometry imaging , workflow , instrumentation (computer programming) , maldi imaging , computer science , focus (optics) , modalities , molecular imaging , image resolution , mass spectrometry , medical physics , computational biology , data science , artificial intelligence , chemistry , medicine , biology , database , physics , chromatography , social science , sociology , optics , operating system , microbiology and biotechnology , adsorption , desorption , organic chemistry , in vivo , matrix assisted laser desorption/ionization
The applicability of mass spectrometry imaging (MSI) has exponentially increased with the improvement of sample preparation, instrumentation (spatial resolution) and data analysis. The number of MSI publications listed in PubMed continues to grow with 378 published articles in 2020‐2021. Initially, MSI was just sensitive enough to identify molecular features correlating with distinct tissue regions, similar to the resolution achieved by visual inspection after standard immunohistochemical staining. Although the spatial resolution was limited compared with other imaging modalities, the molecular intensity mapping added a new exciting capability. Over the past decade, significant improvements in every step of the workflow and most importantly in instrumentation were made, which now enables the molecular analysis at a cellular and even subcellular level. Here, we summarize the latest developments in MSI, with a focus on the latest approaches for tissue‐based imaging described in 2020.