Open AccessPhytochemical profiling of the stem bark of Betula utilis from different geographical regions of India using UHPLC‐ESI‐MS/MS
Author(s) -
Mishra Tripti,
Chandra Preeti,
Kumar Brijesh,
Baleshwar Meena,
Joshi Pushpa,
Rana Tikam Singh,
Upreti Dalip Kumar,
Pal Mahesh
Publication year - 2021
Publication title -
analytical science advances
Language(s) - English
Resource type - Journals
ISSN - 2628-5452
DOI - 10.1002/ansa.202000073
Subject(s) - quadrupole ion trap , phytochemical , triple quadrupole mass spectrometer , betulinic acid , chemistry , bark (sound) , chromatography , mass spectrometry , ion trap , botany , selected reaction monitoring , tandem mass spectrometry , biology , biochemistry , ecology , genetics
A simple, rapid, sensitive, and reliable ultra‐high‐performance liquid chromatography‐hybrid linear ion trap triple quadrupole mass spectrometry method was developed and validated for simultaneous determination of 10 bioactive compounds in stem bark of Betula utilis grown in high altitude of Himalaya, India. The objective of the study is to develop and validate ultra‐high‐performance liquid chromatography‐hybrid linear ion trap triple quadrupole mass spectrometry for investigation of geographical variations of triterpenoids, phenolics, and flavonoids contents in stem bark of B. utilis . The validated method was successfully applied to investigate geographical variations of triterpenoids, phenolics, and flavonoids in stem bark of B. utilis . The contents of betulinic acid and oleanolic acid were detected higher among selected analytes. The present variation study reveals great importance for the application and overall assessment of B. utilis .