Open AccessDemonstration of a new antinuclear antibody (ANTI‐RA33) that is highly specific for rheumatoid arthritis
Author(s) -
Hassfeld Wolfgang,
Steiner Günter,
Hartmuth Klaus,
Kolarz Gernot,
Scherak Othmar,
Graninger Winfried,
Thumb Norbert,
Smolen Josef S.
Publication year - 1989
Publication title -
arthritis & rheumatism
Language(s) - English
Resource type - Journals
eISSN - 1529-0131
pISSN - 0004-3591
DOI - 10.1002/anr.1780321204
Subject(s) - autoantibody , rheumatoid arthritis , rnase p , anti nuclear antibody , rheumatoid factor , antibody , immunology , antigen , medicine , arthritis , biology , biochemistry , rna , gene
Using immunoblot analysis with soluble nuclear extracts from HeLa cells, we identified autoantibodies to an antigen with a molecular weight of ∼33,000 in 36% of 95 sera from rheumatoid arthritis patients, but in only 1 of 170 controls. The antigen, termed RA33, was resistant to DNase and RNase digestion but sensitive to proteinase K treatment. There was no discernible relation to other autoantibodies. Thus, this newly described autoantibody appears to be highly specific for rheumatoid arthritis.