Functional Switch and Ethyl Group Formation in the Bacterial Polytrichastrene Synthase from  Chryseobacterium polytrichastri | Zendy

Hou Anwei | Zendy; Goldfuss Bernd | Zendy; Dickschat Jeroen S. | Zendy

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Functional Switch and Ethyl Group Formation in the Bacterial Polytrichastrene Synthase from Chryseobacterium polytrichastri

Author(s) -

Hou Anwei,

Goldfuss Bernd,

Dickschat Jeroen S.

Publication year - 2021

Publication title -

angewandte chemie international edition

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.831

H-Index - 550

eISSN - 1521-3773

pISSN - 1433-7851

DOI - 10.1002/anie.202109465

Subject(s) - atp synthase , chryseobacterium , chemistry , group (periodic table) , microbiology and biotechnology , biology , biochemistry , enzyme , organic chemistry , gene , 16s ribosomal rna

A reinvestigation of the linalool synthase from Chryseobacterium polytrichastri uncovered its diterpene synthase activity, yielding polytrichastrene A and polytrichastrol A with new skeletons, besides known wanju‐2,5‐diene and thunbergol. The enzyme mechanism was investigated by isotopic labeling experiments and DFT calculations to explain an unusual ethyl group formation. Rationally designed exchanges of active site residues showed major functional switches, resulting for I66F in the production of five more new compounds, including polytrichastrene B and polytrichastrol B, while A87T, A192V and the double exchange A87T, A192V gave a product shift towards wanju‐2,5‐diene.

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